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Thermodynamic studies reveal that MT3 binds Cu + through enthalpy-driven processes and Zn + via entropy-driven mechanisms, forming Cu 4 thiolate clusters

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HFF-1 cells maintained in a T-25 cell culture flask (Corning, United States) at 37C in a 5% (v/v) CO 2 atmosphere and harvested at 90% confluence using 0.12% trypsin-EDTA

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