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Briefly, each primary antibody was incubated for 12 h in a humidified chamber at 37 C, followed by detection using the HRP-conjugated secondary antibody and TSA-dendron-fluorophores (Histova, NEON 7-color IHC Kit for cryosection, 1:100), after which the primary and secondary antibodies were thoroughly eluted in stripping solution (Histova, Abcracker) for 60 min at 37 C

Jansson, J

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Am J Clin Nutr 69 : 467475

Patient selection and protocol design matter more than dose alone

some studies determined dogs could not distinguish between shades of red, yellow, and green, while others have shown the opposite result