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Glutathione is synthesized from three main amino acids, glycine, cysteine and glutamine

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Cellular viability Optimization of Sp concentration and its cytotoxicity parameters were established by treating of 1.5 10 5 HeLa or 1.2 10 5 SHSY-5Y cells with serial concentrations of the Sp (100 M1 mM) for 15 min or conducting a time-course (5 min48 h) using 250 M Sp, followed by MTT assay as per standard protocol (Thermo Fisher Scientific)

Precursor Support and Synergistic Nutrients Many health practitioners now recommend supporting glutathione production through precursor amino acids rather than direct supplementation