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Secondary antibodies include goat anti rabbit IgG Alexa Fluor 488 (1:100, Jackson, ab2338046) and donkey anti-sheep IgG Alexa Fluor 488 (1:500, Abcam, ab150177)

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As a final test for the physiological significance of the 6-AH family blockade of Met-dependent cellular outcomes, we evaluated the ability of D-Nle-Cys-Ile-NH-(CH 2 )5-CONH 2 to inhibit the formation of pulmonary colonies by B16-F10 cells after tail vein injection in mice

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it is the reason why so many clinically observable phenomena remain difficult to predict, stratify, or operationalize