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The theory of the origin of sleep which has gained the widest credence is the one that attributes it to anemia of the brain The idea behind this supposition has been that, as the day draws to an end, the circulatory mechanism becomes fatigued, the vasomotor center exhausted, the tone of the blood vessels deficient, and the energy of the heart diminished, and thus is the circulation to the cerebral arteries lessened. NATURE, May 5th, 1898
Following each experiment, the cells were washed once with PBS (LPS solution CBP007B) and fixed with 10% formalin solution (Sigma HT501128-4L) for 10 min at room temperature
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