In general, the rate of synthesis, regeneration, and recycling of glutathione is determined by three key factors 2 : Cellular levels of cysteineCysteine availability is the rate-limiting step in glutathione production, so not enough cysteine and de novo synthesis of glutathione will slow Enzyme glutamate cysteine ligase (GCL) activity, in part regulated by glutathione feedback inhibition Glutathione depletion due to high levels of oxidative stress, inflammation, or exposure to xenobioticsin these situations, de novo synthesis is upregulated Of course, as an antioxidant, one of the most important roles of glutathione is shielding cells from reactive oxygen species and reactive nitrogen species, but thats not its only job
The other cell types, Huh7.5 and A549, seem to have more active metabolic pathways showing only modest impairment in MTT and CTG in the presence of atpenin A5
Diffusion tensor imaging during recovery from severe traumatic brain injury and relation to clinical outcome: A longitudinal study
PLoS One 3(8):e2946, 2008
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After 24 h, cells were infected with icSARS-CoV-2-mNG 109 at a multiplicity of infection (MOI) = 0.2 for CaCo-2 cells and at an MOI = 0.5 for Calu-3 cells or mock-infected
