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Avoid alcohol for at least 3 hours after injection, and ideally wait 24 hours

Use an electric toothbrush with a two-minute timer

Prior to the experiments, rats were trained for two days in a row using a 5-lane rotarod device (Model 47750, UgoBasile, Italy) that is automated at an increasing velocity starting at 4 till 20 rpm and setting a cutoff time of 3 min for 3 runs daily

Its primary job is to neutralize free radicalsunstable molecules that can cause cellular stress if left unchecked

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