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After 24 h, migrated cells on the upper surface of the filter were removed with a cotton swab, while the cells on the bottom of the filter were fixed with 4% paraformaldehyde at room temperature for 15 min and stained with 3% crystal violet solution for 15 min

The habenula has notably high levels of MOR (medial habenula) [44] and also expresses KOR (lateral habenula) [45], is implicated in aversive states [46] including NPW [20], and modulates DA release [47, 48]