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The choice between them depends on whether appetite control or direct metabolic enhancement is the priority

Manuscript Writing and Revision: CB, JR, JOS, and NL-L

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Sodium at 2,000 to 3,000 mg per day

To avoid interferences due to different expression levels of GFP-constructs among cell population, mean fluorescence intensity was measured at the GM130- or MannII-BFP-positive Golgi region as well as at the total cell area, and ratio values were used to determine the relative Golgi accumulation of the constructs