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The authors analyzed B7-H3 mRNA expression levels in TCGA dataset and reported 77% of primary and recurrent glioblastoma samples presented with high expression

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(PubMed) Laaksonen R, Fogelholm M, Himberg JJ, Laakso J, Salorinne Y

After 24 h, migrated cells on the upper surface of the filter were removed with a cotton swab, while the cells on the bottom of the filter were fixed with 4% paraformaldehyde at room temperature for 15 min and stained with 3% crystal violet solution for 15 min