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The detailed mechanism of cellular copper delivery from GHK-Cu whether the complex is taken up intact, whether copper is transferred at the cell surface, and how the copper is subsequently routed within the cell has been characterized in some detail in the published research but is not fully resolved at the level of all intermediate steps
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The population was plated on solid YP20D medium containing 15 g/L agar and incubated at 30 C for 48 h
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