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Molecular docking was conducted using AutoDock 4.2.3 by obtaining the 3D structure of the protein encoded by the key gene (in PDB format) and the structure of the candidate gut metabolite (in SDF format) for docking score calculation

Since there is no known hazardous threshold for vitamin B12, there is no upper limit

The missing four hydrogens remain in the form of the 2NADH and 2 H + formed in the GAPDH reaction

Without enough glutathione to conjugate and clear the histamine, these inflammatory chemicals recirculate through the body, causing systemic MCAS symptoms like severe brain fog, hives, rapid heart rate (tachycardia), and profound digestive distress

Fly images were taken posterior and lateral from median line regions adjacent to VNC

Applications in the Exploration of Obesity Intervention Strategies and Drug Development Based on its unique mechanism of action and favorable physiological regulatory effects, NNMTi has become an important candidate molecule for the exploration of obesity intervention strategies and the development of antiobesity drugs