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Sometimes oxidant-induced apoptosis may cause ageing in experimented subjects [65].However, it has been investigated through several in vitro studies that reactive oxygen species and free radicals induce lipid peroxidation, protein modifications, base alteration and DNA strand breakage which may lead to ageing [66]
To perform reporter assays, cotransfection of the wt or mut vector, and the control vector psiCHECK-2 vector was carried out using miR-221 mimics or control in DPCs, DSCs, and 293T cells
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Conversely, KPCs can contribute to inflammation by providing metabolites or through the secretion of chemokines and cytokines
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