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If a second primary antibody was used, the cells were incubated either for 2 h at room temperature or overnight at 4C

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Angiogenesis experiments use tube formation assays in human umbilical vein endothelial cells (HUVECs), matrigel plug assays in mice, and VEGF protein quantification via Western blot to characterize BPC-157's vascular effects

10.1016/j.cca.2006.05.010 Clin