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If you think you will be purifying the same exact protein again in the future, then you should rinse your elution buffer out with a few column volumes of deionized water (dIH 2 O), and then store the beads in 20% ethanol until youre ready to use them again

Action Items Explore other potential mechanisms by which Tirzepatide may have indirect effects on the central nervous system, despite the initial concerns about its ability to cross the blood-brain barrier

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Gather: peptide vial (lyophilized powder), bacteriostatic water (BAC water) for injection, a 3 mL drawing syringe with 23G needle for the BAC water, and your insulin syringe for dosing

Drawing 12.5 units accurately requires a high-quality syringe with fine graduations

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