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Subsequently, 1 g of finely ground sample was transferred to a 15 mL Falcon tube containing 3 mL extraction buffer (500 mM TrisHCl, 50 mM EDTA, 700 mM sucrose, 100 mM KCl, 2% -mercaptoethanol and 1 mM phenylmethylsulfonyl fluoride, pH 8)

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Clean reads were aligned to the Bos taurus ribosomal database (Ensembl_release109) using Bowtie (v2.4.4), with no mismatches allowed

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doi: 10.1176/ajp.128.2.158 41 SharifKTiosanoSWatadAComaneshterDCohenADShoenfeldYet al

Similar to what we observed for strep-MBP-GRXC2, GSSG treatment led to lower amounts of strep-MBP-HsGRX1-SG and higher amounts of strep-MBP-HsGRX1-S 2 and additional oxidations of other cysteines