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The membranes were then incubated (overnight, at 4 C) with primary antibody dilutions, followed by incubation (1 h, at 37 C) with the appropriate peroxidase-conjugated secondary IgGs (see Supplementary Table 1)

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Regarding the final pharmacokinetic parameter evaluated, Pgp substrate status, 70% of the compounds, along with the three drugs, were predicted not to be Pgp substrates

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-tocopherols are anchored within the hydrophobic core of the phospholipid cell membrane, and the OH group is oriented toward a hydrophilic environment