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Long story short, three years later my mom passed away very shortly after graduation, which was very, very sad for us

Xie HR, Hu LS, Li GY

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Briefly, after enrichment, FoB were washed and resuspended in mannitol and sucrose-BSA (MAS-B) buffer pH 7.2 (70 mM sucrose, 220 mM Mannitol, 10 mM KH 2 PO 4 , 5 mM MgCl 2 , 2 mM Hepes, 1 mM EGTA, and 0.4% fatty acid-free BSA), and flux measurements were started

Here, the high-affinity copper(II)-binding tripeptide GHK was fused to the N-terminus of a GFP variant and an MBP-FG peptide fusion