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Importantly, researchers found that BPC-157's angiogenic effects were achieved without the tumor-promoting concerns associated with some standard growth factors, suggesting a favorable safety profile for wound healing research applications

The cycle threshold (CT) for the target genes ( SIRT-1 and CYP2E1 ) and the endogenous control GAPDH for each sample were detected and the relative quantitation of SIRT-1 and CYP2E1 mRNA expression levels was estimated by normalizing CT of each gene with the CT of GAPDH in each sample using the 2 CT method (Livak and Schmittgen, 2001)

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While no adjustments were made for multiple comparisons, this approach allowed for a focused examination of the proteins of interest in our study

To address this, we developed a method for the rapid immunopurification of the ER, enabling comprehensive profiling of its proteome and metabolome