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Published 2020 Jul 16

Ichikawa et al

Prior to the analytical SEC runs the column was equilibrated with SEC buffer (25 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM -mercaptoethanol) and protein concentrations adjusted to 1.02.0 mg/mL in same buffer in a final applied volume of 100 L