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For determination of the midpoint potentials E of roGFP variants OxD roGFP or, alternatively, the degree of reduction ReD roGFP = 1 OxD roGFP was plotted against the calculated redox potentials of the respective lipoic acid redox buffers and all data points were fitted to a sigmoidal dose-response curve using GraphPadPrism5 (GraphPad Software, www.graphpad.com)

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The cysteine thiol (-SH) group provides potent nucleophilic and reducing properties, allowing GSH to neutralize electrophiles and free radicals

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Based on the pre-experimental data of the ultimate load in the high dose group, low dose group and PBS group, the calculated minimum sample size needed for the biomechanical experiment was nine