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Oxidative stress markers in plasma were measured before ERCP and after 24 and 48 hours: MDA (malondialdehyde) plasma level (lipids oxidation product) AOPP (advanced oxidation protein products) level Glutathione plasma level (total, and reduced) Several inflammation markers were measured before the ERCP and after 48 hours, in addition to the oxidative stress markers: Fibrinogen level CRP level Transferase saturation level MDA Level Measurement The MDA levels will be measured by a TBARS (Thiobarbituric acid reactive substances) assay using the spectrophotometric method [32]