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Our study makes a valuable contribution to the existing body of knowledge in this area of research from several aspects

3 Cellular signaling pathways of butyrate 3.1 G protein-coupled receptors (GPCRs) Butyrate is the ligand for metabolite-sensing G-protein coupled receptors (GPCRs), mainly GPR43, GPR41, and GPR109a (100), which are also known as free fatty acid receptors 2 (FFAR2), free fatty acid receptors 3 (FFAR3), and Hydroxy-carboxylic acid receptor 2(HCA2), respectively (101, 102)

The methods for evaluating sleepwake behavior in these patients is diverse and can be categorized into subjective and objective/semi-quantitative approaches

Split dosing (250 mcg twice daily vs 500 mcg once daily) produces comparable outcomes in most gut-healing models, though twice-daily administration may smooth plasma concentration curves and reduce transient injection site reactions

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This bypasses the digestive system and ensures a higher absorption rate compared to oral supplements