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doi:10.1016/j.tox.2011.03.001, PMID: 21414382 Valko M, Morris H, Cronin MT (2005) Metals, toxicity and oxidative stress

Protoplasts from barley endosperm A pellet of crude protoplast, obtained at 40-50 g for 2-4 min, was suspended in 2 ml of 40% (w/v) Nycodenz (in 50 mM CaCl2, 25 mM MES, pH 5.5), overlaid with 1 ml each of 26.6%, 13.3% and 6.6% Nycodenz (produced by dilution of the 40% Nycodenz solution with the protoplast medium)

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Science of the Total Environment, 745: 140882 Cheng Q, Liu Z, Huang Y, Li F, Nengzi L, Zhang J (2020)

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