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BH4 is regenerated from its oxidized form by dihydrofolate reductase (DHFR), ensuring its availability as a cofactor

Weidner KM, Behrens J, Vandekerckhove J, Birchmeier W

As such, estimating mBDNF levels by subtracting pro-BDNF from total BDNF, using validated kits, could be another valid alternative strategy that should be further investigated

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