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The ideal parameters for the process were identified as a liquid-to-material ratio of 7:1 (mL/g), a microwave power setting of 400 W, a treatment duration of 4 min, the addition of alkaline protease at a concentration of 0.1% (black sesame powder), a pH level of 8.0, and reaction temperature for enzymes maintained at 50 C

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Phase 2 dose-finding trial (Lancet 2021) showed clear dose-response from 0.3 mg up to 4.5 mg/week, but 2.4 mg is the dose Novo Nordisk advanced into Phase 3 and is the most commonly self-reported community ceiling