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Lactate Dehydrogenase (LDH) Assay The relative amounts of LDH released into the media (conducted in triplicates for each group) were determined using CytoTox-ONE Homogeneous Membrane Integrity Assay kit Cat.#G7892 (Promega, USA) according to the manufacturers instructions

analysis (calcd., found for C 34 H 58 N 14 O 8 ): C (51.63, 51.62), H (7.39, 7.40), O (16.18,16.19), N (24.79, 24.80)

Biological aging involves: Collagen degradation Chronic low-grade inflammation (inflammaging) Reduced cellular repair Muscle decline Connective tissue degeneration Mitochondrial dysfunction Slower wound healing Oxidative stress accumulation GHK-Cu has attracted attention because studies suggest it may influence several of these pathways simultaneously

It has been extensively studied in preclinical and laboratory research for its role in cellular communication, tissue remodeling, and regenerative biochemistry

Preclinical research has demonstrated that GHK-Cu may promote wound healing and skin repair by stimulating collagen and glycosaminoglycan synthesis