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Population E1 was passaged twice and, from this culture, approximately 1.1 10 9 cells were prepared for sorting (assuming 1 OD 600 1 10 7 cells) as described above with 50 M BP labeling for 30 min

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137 The intrinsic reactivity and peculiar chemistry of per-sulphides and poly-sulphides indicate that they might carry out numerous signalling functions that could currently be underestimated mainly owing to the technical difficulties in studying these metabolites in biological milieu

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