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(1999) 276:R15414

Flow cytometric analysis of mitochondrial transmembrane potential ( m ) and mitochondrial mass m was estimated by staining for 15 min at 37 o C in the dark with 1 M tetramethylrhodamine methyl ester (TMRM, ThermoFisher Cat No T668, excitation: 543 nm, emission: 567 nm recorded in FL-2) and 20 nm 3,3-dihexyloxacarbocyanine iodide (DiOC 6 , ThermoFisher Cat No D273, excitation: 488 nm, emission: 525 nm recorded in FL-1)

Every single meal and snack should be built around a core of lean protein