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Once they become too short, cells can no longer divide efficiently, leading to diminished repair capacity and the gradual onset of aging

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MATERIALS AND METHODS PRISMA guideline was used for completing this review which was registered with the PROSPERO database (Code: [removed for blind peer review])

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Due to the ribosomal depletion process in the library preparation protocol and the expected ribosomal sequence reads, counting was performed with a modified annotation file which includes the 45 s ribosome annotation for better accuracy