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Growth media was replaced with binding buffer [phenol-free DMEM containing 25 mM HEPES and 0.1% (w/v) BSA] containing 0.7 nM 125 I-Ex(939) or 0.15 nM 125 I-hGLP-1 and increasing concentrations of unlabelled ligand
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Glutathione is actually required for other antioxidants like vitamin C, E, and Selenium to be properly utilized in the body
doi: 10.1158/1535-7163.MCT-05-0303 95 ClendenenTVLundinEZeleniuch-JacquotteAKoenigKLBerrinoFLukanovaAet al
doi:10.1123/ijspp.2023-0018
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