Physiology, current indications and future directions
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Compound purity variations can significantly impact experimental outcomes
Quantitative PCR Total RNA was isolated from MIN6 cells using RNAiso Plus reagent (Takara Bio Inc., Shiga, Japan), and 2-g aliquots were converted into cDNA using an Applied Biosystems High Capacity cDNA Reverse Transcription Kit (Life Technologies Co, Carlsbad, CA)
doi: 10.1038/s41586-022-05388-4 373 WeerasekeraA.Dost.FeiD
