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The combined protein sample was placed in a 10-kDa cutoff dialysis cassette (66382, Thermo Fisher Scientific) pre-equilibrated with PBS buffer for 5 minutes

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Post hoc pairwise t -tests were conducted to compare the preoperative scores and the postoperative scores

The idea behind it is genuinely elegant: rather than tweaking brain chemicals the way caffeine or a stimulant does, dihexa was supposed to help neurons physically grow new synapses the connection points where brain cells talk to each other

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