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For most of these types of lipidation, alkyne- or azide-modified lipid precursors have been generated that enable metabolic labeling of lipidated proteins and, following CuAAC tagging and then profiling by in-gel fluorescence or affinity enrichment for identification by MS-based proteomics (Figure 66)

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Cruciferous vegetables and their bioactive metabolites: from prevention to novel therapies of colorectal cancer