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Total RNA was isolated from cultured CRC cells or CRC tumor and peritumor tissues and reversely transcribed

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Paired-end reads (DNBSEQ, 2 100 bp) were obtained from three biological replicates from wild type ( Candida parapsilosis CLIB214), met4 , and met4/met28 incubated in the presence or absence of cysteine/methionine, while for met28 two replicates were obtained from one lineage and the third from the other lineage

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524,526,527 Encouragingly, compared to placebo, folic acid improved scores in communication and social interaction, providing promising preliminary evidence for language impairment in children with autism