Other GLP-1 receptor agonists (such as dulaglutide or liraglutide) have different pharmacokinetic profiles and may be better tolerated
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Briefly, 2 10 7 cells were lysed in 600 l of PLB buffer (100 mM KCl, 5 mM MgCl 2 , 10 mM HEPES pH 7.0 and 0.5% NP-40) with RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, 10777019) and protease inhibitor (APExBIO, k1007) and 12 l of lysate was saved as input
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RNA quantity and integrity were analyzed with Nanodrop (Thermo Fisher Scientific, Wilmington, DE) and Nano chip for Eukaryotes on the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA)