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Notably, gemcitabine was not used in this setting, because our preliminary studies had demonstrated that extended exposure to gemcitabine in the 3D setting resulted in a complete loss of cancer cells on the top of the matrices

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Mechanistically, NNMT suppression increased adipose SAM and NAD, upregulated polyamine flux enzymes (ODC, SSAT), and altered histone methylation consistent with a shift toward oxidative metabolism [2]