Both GLP-2 [133] and its DPP4 cleavage product GLP-2 [333] have been detected by HPLC in rat ileal extracts and human plasma (80), and a DPP4 inhibitor (Val-Pyr) completely abolished formation of GLP-2 [333] in human plasma in vitro (81)
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Nomura DK, Morrison BE, Blankman JL, Long JZ, Kinsey SG, Marcondes MC et al
The resulting peptides were reconstituted in 200 L of 0.1% formic acid, desalted using Pierce peptide desalting spin columns (Thermo Fisher Scientific), and eluted with 600 L of 50:49.9:0.1 (acetonitrile/water/formic acid) before being dried completely
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