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DNA dot-blot analysis Genomic DNA from erythroblasts was extracted using phenol:chloroform:isoamyl alcohol (25:24:1) and precipitated with ethanol before dissolving in water

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Alternatively, the affinity matrix can be mixed directly with the protein lysates, agitated for some amount of time, centrifuged and collected in batch selection methods

2008;15(6-7):40815 Sivalingam N, Basivireddy J, Balasubramanian KA, et al

Possible Adverse Effects of High-Dose Nicotinamide: Mechanisms and Safety Assessment