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Fluorescent in situ hybridization ( FISH) and immunohistochemical (IHC) visualization of fluorescent GEP44 (f-Cy5-GEP44) and its localization AP/NTS neurons that express GLP-1R and Y1-R or Y2-R Male Sprague-Dawley rats were briefly anesthetized with isoflurane (5% induction followed by 23% maintenance) to facilitate implantation of an in-dwelling cannula (26 gauge) directed at the 4 th cerebroventricular region (coordinates: on the midline, 2.5 mm anterior to the occipital suture and 5.2 mm ventral to the skull.) Postoperative analgesia (2 mg/kg meloxicam) was administered subcutaneously for two days, and the rats were allowed to recover for one week

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