After reconstitution for laboratory use, keep cold and use promptly
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It was also indicated that the applicability of IM-MS, which provides rotationally averaged CCSs of molecular ions that can be correlated with conformation, to the study of IDPs is further enhanced by the ability of this technique to examine absolute conformation(s), populations of conformation, and conformational changes (Jurneczko et al., 2012)
Shaking the vial creates mechanical stress that can break these chains apart (a process called shearing), destroying the molecule and rendering it ineffective for research