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HPLC purity should be above 98%
Unlike NDH-2 and Ndi1 contain Glu at this position (Glu198 of NDH-2, Glu273 of Ndi1), which interacts with adenine ribose moiety of NAD and thus determines Ndi1 or NDH-2 selectivity for NADH over NADPH 8,25 , cFSP1 contain His173 (His174 for hFSP1) at the corresponding position and would have a different selectivity comparing with Ndi1 and NDH-2
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Remove any air bubbles and then inject into the muscle
RRID:AB_2734118) and mouse anti-goat IgG-HRP (Santa Cruz Biotechnologies, sc-2354
Nakagaki K, et al
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