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After OGD treatment, the number of Ki-67-positive endothelial cells significantly decreased, while the intracellular expression levels of senescence markers P21, P53, and the DNA damage indicator -H2AX were markedly elevated (Figure 3B)

Subsequently, single-cell sequencing data, were preprocessed with StanDep 20 to identify core sets of reactions active in each cell which then were used to identify context-specific metabolic networks that contain those reactions using fastcore 21

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