Trypsin was neutralized with 500 l complete medium, the cells were resuspended and 100 l of the suspension was mixed with 6.9 ml Beckman Coulter Isoton II diluent (Fisher Scientific, NC2470899) for counting with a Beckman Coulter Z1 Coulter Cell Particle counter
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It does not substitute for consultation with a licensed physician or dermatologist about your specific skin concerns
TyrBm activity was determined as explained in the inhibition assay above with varying concentrations of H 2 O 2 , ranging from 0 to 90 mM, while maintaining the concentration of HQ constant
Stored refrigerated until dispatch
