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Comparison conditions were as follows: 0 month, accelerated 6 month test

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Subsequently, the membranes were washed with TBST and incubated with appropriate horseradish peroxidase-linked secondary antibodies for 2 h at room temperature followed by further washing with TBST

Antigen-presenting cells identify the PAMPs via the TLRs family and then activate the intracellular signaling pathway to upregulate the expression of inflammatory cytokines, chemokines, and interferons

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