Samples were directly infused into a Waters Micromass ZQ mass spectrometer, using a 250 L syringe
Tracking doesnt need to be complicated
According to the CDC, nearly 42.4% of American adults are considered obese, a figure that has steadily risen over the years
Interest in the melanocortin system as a target for sexual dysfunction began more than two decades ago, when early studies showed that systemic administration of Melanotan II (MT-II), a synthetic analog of alpha-MSH, induced erections in multiple species, including humans
See Supplementary Table 1 for siRNA target sequences
Tissues were then incubated in 10, 20, and 30% sucrose in 1 PBS, embedded in OCT compound (Tissue-Tek, Sakura Finetek, 4583), snap frozen on dry ice, and then stored frozen at 80C