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Probiotics for the Treatment of Bacterial Vaginosis: A Meta-Analysis

MDA-MB-453 and BT-474 cells were grown in DMEM/F12 media (Gibco) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher), 2 mM L-glutamine (Thermo Fisher), amphotericin B 2.5 g/mL (Gibco), 100 U/mL penicillin/100 g/mL streptomycin (Thermo Fisher) and, only with the BT-474 cell lines, 10 g/ml insulin (Merck) were added

Both aliquots were incubated at 37 C for either 15 min or 2 h

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the evidence that the combination works in humans does not exist

The Q227E mutant had roughly two-fold higher catalytic efficiency with arogenate than with prephenate (0.2 vs