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However, these are not yet recommended in clinical guidelines for prevention or management of CVD (Chen et al., 2002

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After washing (PBS pH 7.4, 1 mM DTT), p97 was eluted (50 mM Tris pH 8.0, 10 mM glutathione) and subjected to GST tag cleavage by HRV3C protease 43

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Any unexpected symptom that develops after initiating the combined protocol should be evaluated in the context of potential compound interactions