He Y, Li S, Jiang L, Wu K, Chen S, Su L, Liu C, Liu P, Luo W, Zhong S, et al
with 100 g TNP-Ficoll FITC (Biosearch Technologies F-1300F) and 30 min later tissues were analyzed with flow cytometry (splenocytes were stained with surface markers for the detection of FoB and MZB, with the addition of biotinylated anti-TNP (BD Pharmingen 554055) + Streptavidin-APC (Biolegend 405207 for TNP detection) or snap-frozen for immunofluorescence microscopy in OCT (Sakura 4583)
Sometimes excess supplemented cysteine, high amounts of sulphur-containing foods (such as eggs, garlic, onions and brassicas), or dietary sulfites might cause or worsen the reaction
These enzymes play central roles in triglyceride hydrolysis and fatty acid metabolism
Proteins were visualized using the Supersignal West Pico Chemiluminescent Substrate system (Pierce, Fenton, MO) and molecular weights determined by comparison to protein ladders MDCK cells were grown to 100% confluency on the coverslips in six-well plates and washed twice with PBS
Identity for lot IP10-260411 is confirmed by HPLC-UV with coupled LC-MS