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For this experiment, the aim is to do concave maximisation, which is simply to search for the optimum Vmax, that can be assumed relevant to the in vitro enzyme level, for the maximum glutathione production

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b , Colour expression of authentic cyanidin ( 8 ) in reaction buffers with different combinations of co-factors and EDTA, showing the formation of the blue iron co-pigment of the anionic quinonoid base ( 8 -AM)